Effects of scalding injury on the components of rabbit paw lymph

A technique for lymph collection has been described. Rabbit paw lymph was collected by this technique before and after scalding injury (75 degrees C for 20 s). The lymph concentrations of zinc, copper, iron, cobalt and lipid peroxide were measured. After scalding, the lymph flow increased rapidly and reached a maximum at 1 h, and then decreased gradually. The lymph concentrations of copper, iron and cobalt increased about two-fold in the first 30 min and the elevated levels persisted until 4 h. However, the increase of zinc was less than that of the other three metals. Total protein concentrations also increased twofold in lymph and the elevated level persisted for 4 h. Using gel filtration, the elution pattern of lymph after scalding was almost the same as that before scalding. Lipid peroxide in lymph increased about three-fold following scalding. However, the plasma concentrations of total protein and lipid peroxide did not change significantly following scalding.