Covalently bonded aptamer-functionalised magnetic mesoporous carbon for high-efficiency chloramphenicol detection

A novel aptamer-modified magnetic mesoporous carbon was prepared to develop a specific and sensitive magnetic solid-phase extraction method through combination with ultra-high performance liquid chromatography-tandem mass spectrometry for the analysis chloramphenicol in complex samples. More specifically, the chloramphenicol aptamer-modified Mg/Al layered double hydroxide magnetic mesoporous carbon was employed as a novel magnetic solid-phase extraction sorbent for analyte enrichment and sample clean-up. The extraction solvent, extraction time, desorption solvent, and desorption time were investigated. It was found that the mesoporous structure and aptamer-based affinity interactions resulted in acceptable selective recognition and a good chemical stability toward trace amounts of chloramphenicol. Upon combination with the ultra-high performance liquid chromatography-tandem mass spectrometry technique, a specific and sensitive recognition method was developed with a low limit of detection (0.94 pmol/L, S/N = 3) for chloramphenicol analysis. The developed method was successfully employed for the determination of chloramphenicol in complex serum, milk powders, fish and chicken samples, giving recoveries of 87.0-107% with relative standard deviations of 3.1-9.7%.