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Allogeneic Limbal Epithelial Transplantation Modified With Solid Platelet-Rich Plasma for Bilateral Limbal Stem Cell
Rosa Alvarado-Villacorta1, Karla Paola García-Carmona1, María Esther Martínez-Pardo2
1Cornea Department, Hospital General de México "Dr. Eduardo Liceaga," Mexico City, Mexico; and.
Cornea
|April 4, 2020
Summary
This study shows a new surgical method for treating severe limbal stem cell deficiency (LSCD) using corneal transplants and platelet-rich plasma (PRP). The combined treatment successfully restored the ocular surface and improved vision in a patient with LSCD.
Area of Science:
- Ophthalmology
- Regenerative Medicine
- Tissue Engineering
Background:
- Bilateral limbal stem cell deficiency (LSCD) severely impairs vision and ocular surface integrity.
- Penicillin-induced Stevens-Johnson Syndrome and lime corneal burns are significant causes of LSCD.
- Current treatments for LSCD have limitations in efficacy and accessibility.
Observation:
- A 59-year-old male patient presented with total LSCD and significantly reduced visual acuity in his right eye following a lime corneal burn.
- The patient had a history of bilateral LSCD attributed to penicillin-induced Stevens-Johnson Syndrome.
- The ocular surface was stabilized before surgical intervention.
Findings:
- An allogeneic limbal epithelial stem cell transplantation was performed using a cadaveric donor.
- Solid activated platelet-rich plasma (PRP) was used to cover the limbal grafts, providing nourishment to the ocular surface microenvironment.
- Complete corneal reepithelialization was observed within one week post-procedure.
- Visual acuity improved from hand motion to 20/70 by three months.
Implications:
- This modified limbal transplantation procedure incorporating solid PRP represents a promising, safe, and effective treatment for ocular surface diseases.
- The technique enhances epithelial wound healing and visual recovery in patients with severe LSCD.
- This is the first reported use of solid PRP in conjunction with limbal transplantation, offering a novel therapeutic approach.
