Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calcium, calmodulin and cell proliferation.

A R Means1, C D Rasmussen

  • 1Department of Cell Biology, Baylor College of Medicine, Houston, Texas.

Cell Calcium
|December 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lactoferrin and lysozyme for Kenyan children presenting with wasting and diarrhea: A 2 × 2 factorial randomized controlled trial.

medRxiv : the preprint server for health sciences·2026
Same author

Effect of arm position on image quality and radiation dose during thorax and abdomen computed tomography scans.

Radiography (London, England : 1995)·2024
Same author

Cardiomyocyte growth regulation by Ca(2+)-calmodulin.

Trends in cardiovascular medicine·2011
Same author

Calmodulin-dependent kinase II mediates vascular smooth muscle cell proliferation and is potentiated by extracellular signal regulated kinase.

Endocrinology·2010
Same author

Physiological roles of the Ca2+/CaM-dependent protein kinase cascade in health and disease.

Sub-cellular biochemistry·2008
Same author

Spermatogenesis and the regulation of Ca(2+)-calmodulin-dependent protein kinase IV localization are not dependent on calspermin.

Molecular and cellular biology·2001
Same journal

Calcium handling properties and arrhythmia vulnerability of cardiomyocytes from dystrophin-deficient mdx mice.

Cell calcium·2026
Same journal

Molecular actors of the Calcium signaling in the endothelial-to-mesenchymal transition: poorly explored therapeutic targets.

Cell calcium·2026
Same journal

High-throughput quantitation of pathogen-induced calcium signals captured through live-cell fluorescence microscopy.

Cell calcium·2026
Same journal

A model for the complete sequence of the human voltage-gated calcium channel (Ca<sub>V</sub>1.2) in a lipid bilayer.

Cell calcium·2026
Same journal

STIM1 but not STIM1L couples Ca<sup>2+</sup> extrusion to SOCE, promoting NFATc1 activation and human myotube growth.

Cell calcium·2026
Same journal

Orai1 mediates I<sub>CRAC</sub> and SOCE in human placental extravillous trophoblasts and contributes to cell viability, migration, and invasion.

Cell calcium·2026
See all related articles

Calmodulin regulates cell cycle progression at G1/S and metaphase transitions. Manipulating calmodulin levels in mouse cells reveals its critical roles in cell division and microtubule stability.

Area of Science:

  • Molecular Biology
  • Cell Biology

Background:

  • Calcium and calmodulin are implicated as regulatory factors in cell cycle progression.
  • Understanding their precise roles requires experimental manipulation of their intracellular levels.

Purpose of the Study:

  • To investigate the regulatory roles of calmodulin in cell cycle progression.
  • To elucidate the molecular mechanisms governing calmodulin's function in cell division.

Main Methods:

  • Generation of clonal mouse cell lines with altered calmodulin expression (overproduction, reduction via antisense RNA, or altered buffering with parvalbumin).
  • Analysis of cell cycle progression, gene expression, and tubulin mRNA levels in response to calmodulin level changes.

Main Results:

Related Experiment Videos

  • Calmodulin acts at the G1/S boundary and metaphase transition.
  • A calmodulin-independent Ca2+ event occurs at mitotic prophase.
  • Elevated calmodulin overrides post-transcriptional regulation, increases G1 gene expression related to growth control, and decreases tubulin mRNA, impacting microtubule stability.

    • Conclusions:

    • Calmodulin plays critical, multi-point roles in regulating the cell cycle.
    • Calmodulin levels influence gene expression and microtubule dynamics.
    • Inducible cell lines provide valuable tools for studying calmodulin's molecular mechanisms in cell cycle control.