Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

Two-Dimensional Microscopy in Microbiology

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Light Harvesting Complex-Like Protein in Maintenance of Photosynthetic Components in <i>Chlamydomonas</i>.

Plant physiology·2017

Same author

[Elevation of red cell distribution width during hospitalization predicts mortality in patients with sepsis].

Zhonghua wei zhong bing ji jiu yi xue·2017

Same author

Transport of nanoparticles across pulmonary surfactant monolayer: a molecular dynamics study.

Physical chemistry chemical physics : PCCP·2017

Same author

Immiscible hydrocarbon fluids in the deep carbon cycle.

Nature communications·2017

Same author

TGF-β1-silenced leukemia cell-derived exosomes target dendritic cells to induce potent anti-leukemic immunity in a mouse model.

Cancer immunology, immunotherapy : CII·2017

Same author

miR-34a knockout attenuates cognitive deficits in APP/PS1 mice through inhibition of the amyloidogenic processing of APP.

Life sciences·2017

Search research articles

Related Experiment Video

Updated: Dec 25, 2025

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Published on: October 29, 2019

Three-Dimensional Single-Molecule Localization Microscopy in Whole-Cell and Tissue Specimens.

Sheng Liu¹, Hyun Huh², Sang-Hyuk Lee^2,3

¹Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907, USA;

Annual Review of Biomedical Engineering

|April 4, 2020

Summary

Super-resolution microscopy achieves nanoscale imaging in thick biological samples. This review covers challenges and solutions for 3D single-molecule localization microscopy in cells and tissues.

Keywords:

CRLB Cramér–Rao lower bound adaptive optics fluorescence microscopy light-sheet microscopy super-resolution microscopy tissue imaging

More Related Videos

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

Author Spotlight: Unraveling Bacterial Responses to Antibiotics and Immune System in Tissues

Author Spotlight: Unraveling Bacterial Responses to Antibiotics and Immune System in Tissues

Published on: March 1, 2024

Related Experiment Videos

Last Updated: Dec 25, 2025

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Published on: October 29, 2019

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

Author Spotlight: Unraveling Bacterial Responses to Antibiotics and Immune System in Tissues

Author Spotlight: Unraveling Bacterial Responses to Antibiotics and Immune System in Tissues

Published on: March 1, 2024

Area of Science:

Biomedical Research
Microscopy
Cell Biology

Background:

Super-resolution microscopy visualizes cellular structures and protein functions.
Thick specimens limit resolution and imaging depth in nanoscopy.
3D single-molecule localization microscopy (SMLM) is crucial for nanoscale imaging.

Purpose of the Study:

Review major roadblocks in 3D SMLM for thick specimens.
Discuss developing solutions for nanoscale imaging in cells and tissues.
Highlight innovations and their trade-offs.

Main Methods:

Focus on 3D single-molecule localization microscopy.
Review challenges: background fluorescence, aberrations, photon information, drift, reconstruction, photobleaching.
Analyze innovative solutions and their core concepts.

Main Results:

Identified key challenges hindering nanoscale resolution in thick samples.
Presented various strategies to overcome limitations in 3D SMLM.
Showcased breakthroughs in resolving thick biological volumes.

Conclusions:

3D SMLM faces significant hurdles in thick specimens.
Ongoing innovations are improving resolution and depth.
Further advancements are needed for comprehensive nanoscale imaging.