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Updated: Dec 25, 2025

Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors
Published on: December 6, 2018
Effects of substrate type on denitrification efficiency and microbial community structure in constructed wetlands
Guiping Fu1, Jinfa Wu1, Jingyi Han1
1Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.
Abstract:
Three constructed wetland systems were established to treat saline sewage via high-porosity ceramsite, activated carbon, and low-porosity sand: A (ceramsite + activated carbon + sand), B (sand + activated carbon + ceramsite), and C (sand). The distribution of dissolved oxygen in these systems varied with different filling methods with the best removal efficiency of ammonium nitrogen and total nitrogen observed in system B (97.4 and 96.2%, respectively). The 16S rDNA amplicon sequencing results showed that all the systems had a high abundance of salt-tolerant denitrifiers, and the filling method significantly impacted denitrifying bacteria (e.g., Vibrio and Planctomyces) in the substrate. System B had more diverse dissolved oxygen conditions than system A and showcased aerobic nitrification-denitrification and anaerobic ammonium oxidation pathways. Therefore, the use of substrates with different porosities can improve the dissolved oxygen supply and enhance nitrogen removal efficiency in constructed wetlands.
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