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Updated: Dec 25, 2025

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
Published on: February 8, 2014
In-Line Holography in Transmission Electron Microscopy for the Atomic Resolution Imaging of Single Particle of
1Istituto per la Microelettronica ed i Microsistemi, Consiglio Nazionale delle Ricerche (CNR-IMM), Sezione di Lecce, Campus Universitario, via per Monteroni, 73100 Lecce, Italy.
Abstract:
In this paper, for the first time it is shown how in-line holography in Transmission Electron Microscopy (TEM) enables the study of radiation-sensitive nanoparticles of organic and inorganic materials providing high-contrast holograms of single nanoparticles, while illuminating specimens with a density of current as low as 1-2 e-Å-2s-1. This provides a powerful method for true single-particle atomic resolution imaging and opens up new perspectives for the study of soft matter in biology and materials science. The approach is not limited to a particular class of TEM specimens, such as homogenous samples or samples specially designed for a particular TEM experiment, but has better application in the study of those specimens with differences in shape, chemical composition, crystallography, and orientation, which cannot be currently addressed at atomic resolution.
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