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The sequence around the phosphorylation site of the porcine heart type phosphorylase isoenzyme
V Dombrádi1, A C Willis, G Vereb
1Institute of Medical Chemistry, University School of Medicine, Debrecen, Hungary.
Abstract:
1. A tetradecapeptide containing the phosphorylation site was obtained from 32P-labelled pig heart phosphorylase a isoenzyme by alpha-chymotryptic digestion. 2. The peptide was purified by Mono S cation-exchange chromatography and reversed-phase HPLC. 3. The phosphorylated residue was identified as Ser and the sequence was determined: T D G E R R K Q I S V R G L. 4. The sequence was compared to the known sequences of muscle and liver type isophosphorylases and the structural consequences of the amino acid residue exchanges were predicted.