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Updated: Dec 25, 2025

Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells

Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells

Published on: March 2, 2017

Stem cell-derived polarized hepatocytes.

Viet Loan Dao Thi^1,2, Xianfang Wu³, Rachel L Belote^4,5

¹Laboratory of Virology and Infectious Diseases, The Rockefeller University, New York, NY, USA. VietLoan.DaoThi@med.uni-heidelberg.de.

Nature Communications

|April 5, 2020

Summary

This summary is machine-generated.

Researchers developed a new method using transwell filters to create polarized human stem cell-derived hepatocyte-like cells (HLCs). These polarized HLCs mimic in vivo liver functions, enabling better studies of liver biology and drug metabolism.

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Area of Science:

Hepatology
Stem Cell Biology
Cell Polarity

Background:

Human stem cell-derived hepatocyte-like cells (HLCs) are valuable for liver research.
A key limitation of current HLCs is their lack of in vivo cell polarity.

Purpose of the Study:

To develop a protocol for generating polarized HLCs.
To characterize the in vivo-like functions of these polarized HLCs.

Main Methods:

Utilized transwell filters in a stem cell differentiation protocol.
Generated columnar polarized HLCs with distinct apical and basolateral membranes and tight junctions.

Main Results:

Polarized HLCs exhibit directional secretion of albumin, urea, lipoproteins (basolateral), and bile acids (apical).
Demonstrated recapitulation of hepatitis E virus (HEV) infectious cycle, with directional secretion of viral particles.
Established proof-of-concept for using polarized HLCs in pharmacokinetic and drug-drug interaction studies.

Conclusions:

This novel system generates physiologically relevant polarized HLCs.
Offers a powerful tool for studying hepatocyte biology, liver diseases, and drug metabolism.