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Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
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MicroRNA-331 and microRNA-151-3p as biomarkers in patients with ST-segment elevation myocardial infarction

Martin Horváth1, Veronika Horváthová2,3, Petr Hájek4

  • 1Department of Cardiology, Charles University, 2nd Faculty of Medicine and Motol University Hospital, Prague, Czech Republic. martin@horvath.cz.

Scientific Reports
|April 5, 2020
PubMed

Insights

Plasma microRNAs (miRs) like miR-331 and miR-151-3p show promise as early biomarkers for ST-segment-elevation myocardial infarction (STEMI), potentially indicating vulnerable plaque rupture before cardiac necrosis markers rise.

Area of Science:

  • Cardiovascular Medicine
  • Molecular Biology
  • Biomarker Discovery

Background:

  • ST-segment-elevation myocardial infarction (STEMI) is often caused by vulnerable plaque (VP) rupture.
  • Identifying early biomarkers for STEMI is crucial for timely intervention.
  • Peripheral blood microRNAs (miRs) are being investigated as potential diagnostic markers.

Purpose of the Study:

  • To analyze plasma levels of peripheral blood miRs as biomarkers for type-1 myocardial infarction (STEMI) resulting from VP rupture.
  • To identify specific miRs that can differentiate STEMI patients from control groups.
  • To assess the potential of these miRs as early indicators of myocardial infarction.

Main Methods:

  • Compared plasma miR levels in 20 STEMI patients with controls (no coronary atherosclerosis-NCA and stable coronary artery disease-SCAD).
  • Utilized large-scale miR assay screening to select candidate miRs.
  • Quantified selected miRs (miR-331, miR-151-3p) and performed ROC analysis for biomarker suitability.

Main Results:

  • Two study miRs, miR-331 and miR-151-3p, significantly distinguished STEMI patients from control groups.
  • ROC analysis confirmed the suitability of miR-331 and miR-151-3p as biomarkers.
  • These miRs were elevated early in STEMI, even before cardiac troponin I and other necrosis markers.

Conclusions:

  • miR-331 and miR-151-3p are potential novel biomarkers for STEMI.
  • Their early detection suggests a possible origin in vulnerable plaque rupture.
  • These findings may aid in the early diagnosis of myocardial infarction related to plaque instability.

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