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Related Experiment Video

Updated: Dec 25, 2025

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology
09:45

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Spectrally Combined Encoding for Profiling Heterogeneous Circulating Tumor Cells Using a Multifunctional

Ling-Ling Wu1, Zhi-Ling Zhang1, Man Tang1

  • 1College of Chemistry and Molecular Sciences, The Institute for Advanced Studies, Wuhan University, Wuhan, 430072, P. R. China.

Angewandte Chemie (International Ed. in English)
|April 5, 2020
PubMed
Summary

This study introduces a new spectrally combined encoding (SCE) method for detailed analysis of single circulating tumor cells (CTCs). This technique enhances cancer management by enabling precise, multiplex biomarker profiling of heterogeneous CTCs.

Keywords:
circulating tumor cellcombined encodingmagnetic tagmicrofluidicsoptical encoding

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Area of Science:

  • Biomedical Engineering
  • Cancer Research
  • Analytical Chemistry

Background:

  • Comprehensive phenotypic profiling of heterogeneous circulating tumor cells (CTCs) at single-cell resolution is crucial for effective cancer management.
  • Existing methods face challenges in accurately analyzing the diverse characteristics of individual CTCs.

Purpose of the Study:

  • To develop a novel spectrally combined encoding (SCE) strategy for multiplex biomarker profiling of single CTCs.
  • To utilize a multifunctional nanosphere-mediated microfluidic platform for enhanced CTC analysis.

Main Methods:

  • Proposed a spectrally combined encoding (SCE) strategy using multifunctional nanosphere barcodes with identical magnetic tags and distinct optical signatures.
  • Developed a microfluidic platform for efficient isolation (over 91.6%) of heterogeneous CTCs.
  • Integrated ordered microstructures on-chip for trapping individual CTCs and obtaining composite single-cell spectral signatures.

Main Results:

  • Achieved efficient isolation of heterogeneous CTCs.
  • Enabled in situ spectrally combined encoding (SCE) of CTC phenotypes.
  • Obtained reliable spectral-readout for multiplex biomarker profiling of individual CTCs.

Conclusions:

  • The SCE strategy demonstrates significant potential for multiplex profiling of heterogeneous CTC phenotypes.
  • This approach offers new avenues for advancing cancer research and precision medicine.