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Updated: Dec 25, 2025

Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors
Published on: December 6, 2018
Elemental sulfur-based autotrophic denitrification in stoichiometric S0/N ratio: Calibration and validation of a
C Huiliñir1, L Acosta1, D Yanez1
1Laboratorio de Biotecnología Ambiental, Departamento de Ingeniería Química, Universidad de Santiago de Chile, Av. Lib. Bdo. O Higgins 3363, Santiago, Chile.
Abstract:
The inclusion of S0 hydrolysis in a kinetic model of autotrophic denitrification has been recently proposed; however the model has not been calibrated or validated yet. Thus, a new methodology was developed and applied to calibrate and validate this kinetic model for the first time. An inoculum adapted from a poultry wastewater treatment plant at stoichiometric S0/NO3- ratio was used. The model was calibrated with batch data (initial nitrate concentrations of 50 and 6.25 mg NO3--N/L) at an S0/N ratio = 2.29 mg S/mg N and validated with seven different batch data. The sensitivity analysis showed that the most sensitive parameters are related to S0 hydrolysis. The kinetic model was successfully calibrated with the new methodology and validated, with Theil inequality coefficient values lower than 0.21. Thus, the proposed model and methodology were proved to be well suited for the simulation of elemental sulfur-based autotrophic denitrification in batch systems.
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