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A phosphate-repressible acid phosphatase gene from Aspergillus niger: its cloning, sequencing and transcriptional
W D MacRae1, F P Buxton, S Sibley
1Allelix Inc., Mississauga, Ont., Canada.
Gene
|November 30, 1988
Abstract:
The cloning and sequencing of an Aspergillus niger gene encoding a secreted form of phosphate-repressible acid phosphatase by complementation of a pacA (phosphate-repressible acid phosphatase) mutant of Aspergillus nidulans is described. The gene contains two introns, 201 and 265 nt in length, and codes for a 1.6-kb transcript. Both phosphate concentration and pH of the growth medium affect the level of expression of the gene in A. niger. Similar regulation is observed in A. nidulans transformants. A putative signal peptide, resembling known signal sequences of yeast, is identified.