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Updated: Jul 16, 2026
Rapid Identification of Chemical Genetic Interactions in Saccharomyces cerevisiae
Published on: April 5, 2015
Disruption of RIM15 confers an increased tolerance to heavy metals in Saccharomyces cerevisiae
1Department of Food Science and Technology, Jungwon University, 85, Munmu-ro, Goesan-eup, Goesan-gun, Chungbuk, 367-805, Republic of Korea. hyun1006@jwu.ac.kr.
Objective:
The aim of this study was to identify genes related to a heavy metal tolerance and to elucidate the tolerance mechanism in a eukaryote model using Saccharomyces cerevisiae.
Results:
In this study, one strain tolerant to up to 50 μM Pb(NO3)2 and 30 μM CdCl2 was isolated by screening a transposon-mediated mutant library and the disrupted gene was determined to be RIM15. In addition, this gene's characteristics related to heavy metals-tolerance was proved by deletion and overexpressing of this corresponding gene. The transposon mutant grew faster than the control strain and showed an obvious reduction in the intracellular level of reactive oxygen species (ROS) with activation of MSN4 and CTT1 in YPD medium containing 50 μM Pb(NO3)2 and 30 μM CdCl2 respectively.
Conclusions:
Disruption of RIM15 in S. cerevisiae results in increased tolerance to heavy metal stress.
