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CRISPR as a tool in tumor therapy: A short review

Jiaqi Tan1, Wen Yu1

  • 1Department of Pediatrics Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

Biotechnology and Applied Biochemistry
|April 6, 2020
PubMed

Insights

Gene therapy using the Clustered regulatory interspaced short palindromic repeat (CRISPR) system offers a promising approach for tumor treatment. This review explores CRISPR applications in modifying tumor cells and engineering drugs, highlighting its potential and challenges.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biotechnology

Background:

  • Tumors represent a significant global health challenge.
  • Gene therapy is an emerging and effective strategy for cancer treatment.
  • The Clustered regulatory interspaced short palindromic repeat (CRISPR) system provides precise gene-editing capabilities.

Purpose of the Study:

  • To review the applications of the CRISPR system in tumor therapy.
  • To explore CRISPR's role in gene knockout and gene knock-in strategies.
  • To summarize delivery methods and application areas of CRISPR in oncology.

Main Methods:

  • Review of existing literature on CRISPR technology in cancer research.
  • Analysis of CRISPR applications in tumor cell modification.
  • Evaluation of CRISPR's use in drug engineering for cancer treatment.

Main Results:

  • CRISPR systems, including CRISPRa, are utilized for gene knockout and knock-in in tumor therapy.
  • Applications span tumor cell modification and the engineering of therapeutic drugs.
  • Various delivery approaches are employed for CRISPR-based cancer treatments.

Conclusions:

  • The CRISPR system presents a powerful tool for advancing tumor therapy.
  • Key applications include direct tumor cell modification and innovative drug development.
  • Addressing the challenges associated with CRISPR delivery and efficacy is crucial for clinical translation.
Keywords:
CRISPRgene therapytumor

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