Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Surface Appendages of Archaea01:23

Surface Appendages of Archaea

474
Archaeal surface appendages are highly specialized structures essential for environmental adaptation, encompassing roles in adhesion, biofilm formation, and motility. Among these appendages, pili and archaella stand out for their distinct morphologies and functionalities, enabling archaea to thrive in diverse and often extreme environments.Pili: Adhesion and Biofilm FormationPili are filamentous structures assembled from pilin protein subunits, primarily contributing to adhesion and biofilm...
474
Directing Proteins to the Rough Endoplasmic Reticulum01:34

Directing Proteins to the Rough Endoplasmic Reticulum

16.4K
The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
16.4K
Outer Layers of the Cell Envelope01:18

Outer Layers of the Cell Envelope

781
The outermost layers of prokaryotic cells play a critical role in their survival, virulence, and interaction with the environment. These layers, often composed of polysaccharides, polypeptides, or proteins, form protective and adhesive structures that vary in organization and function.Capsules and Slime LayersCapsules are highly organized, tightly bound layers that firmly attach to the bacterial cell wall. Capsules are usually made of polysaccharides, though some are made of polypeptides. These...
781
Surface Membrane Barriers01:18

Surface Membrane Barriers

2.5K
The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
2.5K
Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

9.2K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
9.2K
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

6.4K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
6.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative evaluation of urine, whole blood, and serum as biological matrices for human biomonitoring of neonicotinoid insecticides and metabolites.

Journal of hazardous materials·2026
Same author

[Trends in burden of osteoporosis in China from 1990 to 2023 and analysis of epidemiological factors].

Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery·2026
Same author

Effects of nutrient forms on the bloom dynamics and phytoplankton community associated with Prorocentrum cordatum.

Harmful algae·2026
Same author

Bacterial Cofactors for CRISPR Activation.

Biochemistry·2026
Same author

LedX: A novel blood-based early lung cancer detection approach with exceptional accuracy and robustness.

Chinese medical journal·2026
Same author

Protocol for Discovery and Characterization of Miniature Cas12 Systems.

ACS chemical biology·2026

Related Experiment Video

Updated: Dec 25, 2025

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
07:10

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Published on: February 19, 2019

9.3K

Structural Basis of Staphylococcus aureus Surface Protein SdrC.

Yishuang Pi1,2, Weizhong Chen1, Quanjiang Ji1,3

  • 1School of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, China.

Biochemistry
|April 7, 2020
PubMed
Summary

Staphylococcus aureus surface protein SdrC uses calcium ions to bind itself, forming biofilms essential for infection. This study reveals the molecular mechanism behind this crucial interaction.

More Related Videos

Development and Assessment of Intracellular Infection Models for Staphylococcus aureus
08:32

Development and Assessment of Intracellular Infection Models for Staphylococcus aureus

Published on: January 17, 2025

1.1K
Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR
08:51

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR

Published on: November 4, 2016

10.1K

Related Experiment Videos

Last Updated: Dec 25, 2025

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
07:10

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Published on: February 19, 2019

9.3K
Development and Assessment of Intracellular Infection Models for Staphylococcus aureus
08:32

Development and Assessment of Intracellular Infection Models for Staphylococcus aureus

Published on: January 17, 2025

1.1K
Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR
08:51

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR

Published on: November 4, 2016

10.1K

Area of Science:

  • Microbiology
  • Structural Biology
  • Infectious Diseases

Background:

  • Staphylococcus aureus surface proteins are critical for host colonization, biofilm formation, and virulence.
  • The SdrC protein mediates bacterial biofilm formation through intermolecular homophilic interactions of its N2 domains.
  • The precise molecular mechanism underlying SdrC's homophilic interaction remains unelucidated.

Purpose of the Study:

  • To elucidate the molecular mechanism of homophilic interaction mediated by the SdrC surface protein from Staphylococcus aureus.
  • To investigate the structural basis for SdrC-mediated biofilm formation.

Main Methods:

  • Determination of two crystal structures of the SdrC N2N3 domains.
  • Analysis of potential homophilic interaction mechanisms, including Ca2+-mediated chelation and domain-domain interactions.
  • Site-directed mutagenesis of key Ca2+-chelating residues in the N2 domain.

Main Results:

  • Two potential homophilic interaction mechanisms were identified: Ca2+-mediated metal chelation between N2 domains and N2-N3 domain interactions.
  • The N3 domain is not essential for biofilm formation induction, suggesting the N2 domain-mediated mechanism is primary.
  • Mutagenesis of Ca2+-chelating residues in the N2 domain impaired protein dimer formation, confirming the role of metal chelation.

Conclusions:

  • The study reveals a likely mechanism for SdrC homophilic interaction involving Ca2+-mediated metal chelation of the N2 domains.
  • This finding provides structural insights into SdrC function in Staphylococcus aureus biofilm formation.
  • Understanding this mechanism opens avenues for developing targeted anti-virulence strategies.