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DHRS12 inhibits the proliferation and metastasis of osteosarcoma via Wnt3a/β-catenin pathway

Zhixian Xu1, Wubing He1, Tie Ke1

  • 1Department of Emergency Surgery, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou, Fujian 350001, PR China.

Future Oncology (London, England)
|April 7, 2020
PubMed

Insights

Overexpression of DHRS12 inhibits osteosarcoma (OS) progression by reducing cell proliferation, migration, and invasion while inducing apoptosis. This occurs through the Wnt3a/β-catenin pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Osteosarcoma (OS) is a primary bone malignancy with limited treatment options.
  • The role of DHRS12 in OS progression requires further elucidation.

Purpose of the Study:

  • To investigate the biological effects of DHRS12 on osteosarcoma (OS).
  • To explore the potential of DHRS12 as a therapeutic target in OS.

Main Methods:

  • Immunohistochemical analysis to assess endogenous DHRS12 expression in OS.
  • Plasmid transfection to overexpress DHRS12 in MG-63 and HOS cell lines.
  • Assays for cell proliferation, invasion, migration, and apoptosis, complemented by Western blot analysis.

Main Results:

  • DHRS12 expression was significantly downregulated in OS tissues.
  • Overexpression of DHRS12 suppressed proliferation, migration, and invasion of OS cells.
  • DHRS12 overexpression induced apoptosis by upregulating Bax, Caspase 9, and Caspase 3, and inactivated the Wnt3a/β-catenin pathway.

Conclusions:

  • DHRS12 exhibits tumor-suppressive properties in osteosarcoma.
  • Overexpression of DHRS12 inhibits OS progression by modulating the Wnt3a/β-catenin signaling pathway.
Keywords:
DHRS12Wnt3a/β-catenininvasionosteosarcomaproliferation

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