Amycolatopsis nivea sp. nov., isolated from a Yellow River sample

A novel actinobacterium, designated strain CFH S0261^T, was isolated from a sediment sample of the Yellow River. The taxonomic position of the strain was investigated by using a polyphasic approach. Cells of strain CFH S0261^T were Gram-reaction-positive, aerobic, non-motile. Growth occurs at 15-37 °C, pH 6.0-8.0 and with 0-9.0 % (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CFH S0261^T was a member of the genus Amycolatopsis. The 16S rRNA gene sequence similarity indicated that strain CFH S0261^T is most closely related to the type strains of Amycolatopsis niigatensis LC11^T (98.95 %), Amycolatopsis echigonensis LC2^T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139^T (98.73 %). The whole-genome of CFH S0261^T showed a G+C content of 69.5 mol%. The ANI values and in silico DDH values between CFH S0261^T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %). The cell wall diamino acid in the peptidoglycan of strain CFH S0261^T was meso-diaminopimelic acid and the whole-cell hydrolysate comprised arabinose, galactose, glucose, rhamnose and ribose. The predominant menaquinone was MK-9(H₄). The major cellular fatty acids were C_16 : 0, iso-C_15 : 0 and iso-C_16 : 0. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and four unidentified glycolipids. On the basis of phenotypic, genotypic and phylogenetic data, strain CFH S0261^T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis nivea sp. nov. is proposed. The type strain is CFH S0261^T (=KCTC 39515^T =CCTCC AA 2014028^T).