Emerging design strategies for constructing multiplex lateral flow test strip sensors.

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Biosensing Technology

Background:

• Conventional lateral flow test strips (LFTS) suffer from single-target detection limitations, impacting accuracy and reliability.
• The need for simultaneous determination of multiple analytes drives the development of advanced sensing technologies.
• High-throughput and multiplexed LFTS are emerging as crucial tools for rapid on-site diagnostics.

Purpose of the Study:

• To systematically review recent achievements in the design, development, and application of multiplexed LFTS sensors.
• To focus on strategies for increasing multiplexing capacity to enhance analytical efficiency and precision.
• To critically analyze the advantages and disadvantages of various multiplexed LFTS approaches.

Main Methods:

• Systematic literature review of multiplexed lateral flow test strip technologies.
• Analysis of design strategies for enhanced multiplexing capacity.
• Presentation and critical evaluation of typical proof-of-concept examples.

Main Results:

• Emerging design strategies significantly increase multiplexing capacity in LFTS.
• Multiplexed LFTS demonstrate improved analytical efficiency and precision for on-site diagnostics.
• Several typical examples showcase the practical application and potential of these advanced sensors.

Conclusions:

• Multiplexed LFTS represent a significant advancement over conventional single-target sensors.
• Further development is needed to address current challenges and fully realize the potential of these technologies.
• Future perspectives include continued innovation in design and broader application in rapid diagnostics.