Related Experiment Video
Updated: Dec 24, 2025
Rat Model of the Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy ALPPS Procedure
Published on: August 14, 2017
RNA-Seq transcriptome profiling in three liver regeneration models in rats: comparative analysis of partial
Dilek Colak1, Olfat Al-Harazi2, Osama M Mustafa2
1Biostatistics, Epidemiology, and Scientific Computing Department, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia. dkcolak@gmail.com.
This study compared gene expression in rat livers after three surgeries: partial hepatectomy (PH), portal vein ligation (PVL), and associated liver partition and portal vein ligation for staged hepatectomy (ALPPS). Time significantly impacts gene expression, with ALPPS showing early similarities to PH.
Area of Science:
- Regenerative medicine
- Molecular biology
- Surgical innovation
Background:
- The liver's remarkable regeneration capacity is crucial for recovery after injury.
- Distinct surgical procedures like partial hepatectomy (PH), portal vein ligation (PVL), and associated liver partition and portal vein ligation for staged hepatectomy (ALPPS) yield different clinical outcomes.
- These clinical variations suggest underlying differences in gene activation and regeneration pathways.
Purpose of the Study:
- To conduct a comprehensive comparative transcriptomic analysis of gene regulation in regenerating rat livers.
- To investigate temporal gene expression patterns at 24 hours and 96 hours post-PH, PVL, and ALPPS.
- To identify unique and overlapping molecular mechanisms driving liver regeneration after different surgical interventions.
Main Methods:
- Comparative transcriptomic analysis of regenerating rat livers.
- Temporal sampling at 24 hours and 96 hours after PH, PVL, and ALPPS.
- Functional pathway, upstream regulator, and network analyses.
Main Results:
- Time is a critical determinant of gene expression changes during liver regeneration.
- Early gene expression profiles of ALPPS were more similar to PH than PVL.
- Common early transcriptomic changes across all procedures involved cell cycle, transcription factors, DNA replication, and signaling pathways (e.g., ATM, p53).
Conclusions:
- Each surgical procedure exhibits unique and overlapping molecular signatures and regenerative pathways.
- Understanding these molecular differences enhances our knowledge of liver regeneration regulators.
- This research can inform patient selection for specific surgical procedures to optimize regenerative outcomes.
