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Updated: Dec 24, 2025

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

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Published on: June 16, 2021

Probing lasting cryoinjuries to oocyte-embryo transcriptome.

Binnur Eroglu¹, Edyta A Szurek¹, Peter Schall²

¹Department of Neuroscience and Regenerative Medicine, Medical College of Georgia/Augusta University, Augusta, GA, United States of America.

|April 7, 2020

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Standard slow freezing of oocytes impacts embryonic gene expression, altering genes involved in mitochondrial function and cellular stress responses. This research identifies key cryoinjuries to improve oocyte cryopreservation safety and success rates.

Area of Science:

Reproductive Biology
Developmental Biology
Genomics

Background:

Oocyte cryopreservation offers fertility preservation for cancer patients and delayed childbearing.
Current methods like vitrification and slow freezing have limitations including biosafety risks and low success rates.

Purpose of the Study:

To investigate the effects of standard slow oocyte cryopreservation on the embryonic genome.
To identify specific cryoinjuries to the transcriptionally active genome in mouse oocytes.

Main Methods:

Mouse metaphase II (MII) oocytes were subjected to standard slow freezing.
Oocytes were analyzed at the four-cell stage post-embryonic genome activation.
Gene expression analysis using microarray and RT-PCR compared to non-frozen controls.

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Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish

Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish

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Related Experiment Videos

Last Updated: Dec 24, 2025

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Published on: June 16, 2021

Author Spotlight: Understanding DNA Damage Response in Mammalian Oocytes and Preimplantation Embryos

Author Spotlight: Understanding DNA Damage Response in Mammalian Oocytes and Preimplantation Embryos

Published on: June 23, 2023

Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish

Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish

Published on: April 22, 2017

Main Results:

Cryopreserved oocytes resulted in embryos with 200 upregulated and 105 downregulated genes.
Affected genes are associated with mitochondrial function, protein ubiquitination, stress response, lipid metabolism, and cell cycle.
Identified previously unrecognized effects of slow freezing on embryonic gene expression.

Conclusions:

Standard slow oocyte freezing induces significant changes in embryonic gene expression.
Understanding these cryoinjuries can guide the development of safer and more effective oocyte cryopreservation techniques.