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Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases
Published on: January 1, 2016
Isolation and characterization of novel indigenous facultative quorum quenching bacterial strains for ambidextrous
Syed Salman Ali Shah1, Kwang-Ho Choo2
1Department of Environmental Engineering, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu 41566, Republic of Korea.
Abstract:
Quorum quenching (QQ), the disruption of microbial communication, has proven to be effective as an innovative anti-biofouling strategy for membrane bioreactors (MBRs). However, QQ bacteria for anaerobic environments have not been extensively analyzed in previous research. This study thus investigated facultative QQ bacterial strains that exhibit potential for use in aerobic and anaerobic MBRs. Two novel QQ strains from the genus Pseudomonas (KS2 and KS10) were isolated from anaerobic digester sludge using signal molecules as the sole carbon source. The two QQ strains exhibited significant signal molecule degradation depending on the oxygen levels and demonstrated endogenous QQ activity, with KS2 producing lactonase and KS10 producing acylase. The QQ strains significantly reduced the formation of the biofilm generated by both Pseudomonas aeruginosa (PAO1) and real sludge. Facultative QQ strains have the potential to offer a more flexible option for effective biofouling control in both aerobic and anaerobic MBRs.
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