Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Indicators02:39

Indicators

57.0K
Certain organic substances change color in dilute solution when the hydronium ion concentration reaches a particular value. For example, phenolphthalein is a colorless substance in any aqueous solution with a hydronium ion concentration greater than 5.0 × 10−9 M (pH < 8.3). In more basic solutions where the hydronium ion concentration is less than 5.0 × 10−9 M (pH > 8.3), it is red or pink. Substances such as phenolphthalein, which can be used to determine the pH of a solution, are...
57.0K
Strong Acid and Base Solutions03:22

Strong Acid and Base Solutions

34.9K
A strong acid is a compound that dissociates completely in an aqueous solution and produces a concentration of hydronium ions equal to the initial concentration of acid. For example, 0.20 M hydrobromic acid will dissociate completely in water and produces 0.20 M of hydronium ions and 0.20 M of bromide ions.
34.9K
pH Scale02:41

pH Scale

78.0K
Hydronium and hydroxide ions are present both in pure water and in all aqueous solutions, and their concentrations are inversely proportional as determined by the ion product of water (Kw). The concentrations of these ions in a solution are often critical determinants of the solution’s properties and the chemical behaviors of its other solutes. Two different solutions can differ in their hydronium or hydroxide ion concentrations by a million, billion, or even trillion times. A common means of...
78.0K
Weak Base Solutions03:21

Weak Base Solutions

24.5K
Some compounds produce hydroxide ions when dissolved by chemically reacting with water molecules. In all cases, these compounds react only partially and so are classified as weak bases. These types of compounds are also abundant in nature and important commodities in various technologies. For example, global production of the weak base ammonia is typically well over 100 metric tons annually, being widely used as an agricultural fertilizer, a raw material for chemical synthesis of other...
24.5K
Determining the pH of Salt Solutions04:08

Determining the pH of Salt Solutions

46.3K
The pH of a salt solution is determined by its component anions and cations. Salts that contain pH-neutral anions and the hydronium ion-producing cations form a solution with a pH less than 7. For example, in ammonium nitrate (NH4NO3) solution, NO3− ions do not react with water whereas NH4+ ions produce the hydronium ions resulting in the acidic solution.  In contrast, salts that contain pH-neutral cations and the hydroxide ion-producing anions form a solution with a pH greater than 7. For...
46.3K
Titration Calculations: Strong Acid - Strong Base02:28

Titration Calculations: Strong Acid - Strong Base

33.5K
Calculating pH for Titration Solutions: Strong Acid/Strong Base
A titration is carried out for 25.00 mL of 0.100 M HCl (strong acid) with 0.100 M of a strong base NaOH. The pH at different volumes of added base solution can be calculated as follows:
(a) Titrant volume = 0 mL. The solution pH is due to the acid ionization of HCl. Because this is a strong acid, the ionization is complete and the hydronium ion molarity is 0.100 M. The pH of the solution is then:
33.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cycle-dependent variation of tumor absorbed dose rates in<sup>177</sup>Lu-DOTATATE therapies.

Biomedical physics & engineering express·2026
Same author

Beyond the tumor: recurrence-prone radiomics for prognostication in negative PSMA PET/CT scans of prostate cancer.

Biomedical physics & engineering express·2026
Same author

Microenvironment at a Distance: Multi-Endocrine-Organ Radiomics to Identify Systemic Signatures in PSMA-Negative Prostate Cancer.

Cancers·2026
Same author

Morpho-phenological and pomological characteristics in differentiating and selecting superior quince (Cydonia oblonga mill.).

BMC plant biology·2026
Same author

Dealing with Ionic Strength Changes in Chemical Kinetics, Including Extrapolation to Zero Ionic Strength.

The journal of physical chemistry. A·2026
Same author

Investigating the effect of runoff characteristics on fatigue cracking in HMA modified with nano-hydrated lime using mechanical and thermodynamic approaches.

Scientific reports·2025

Related Experiment Video

Updated: Dec 24, 2025

Measuring Phagosome pH by Ratiometric Fluorescence Microscopy
14:39

Measuring Phagosome pH by Ratiometric Fluorescence Microscopy

Published on: December 7, 2015

14.3K

Generalized indicator-based determination of solution pH.

Zahra Rasouli1, Hamid Abdollahi2, Marcel Maeder3

  • 1Department of Chemistry, Institute for Advanced Studies in Basic Sciences, P.O. Box 45195-1159, Zanjan, Iran; Department of Chemistry, University of Newcastle, NSW, 2308, Australia.

Analytica Chimica Acta
|April 8, 2020
PubMed
Summary
This summary is machine-generated.

This study introduces a new method for determining pH using mixtures of pH indicators, offering accurate measurements across a wide pH range. The approach is validated for both static solutions and dynamic reactions, like CO2 in amine solutions.

Keywords:
Law of mass actionpHpH determinationpH indicators

More Related Videos

Optimized Procedure for Determining the Adsorption of Phosphonates onto Granular Ferric Hydroxide using a Miniaturized Phosphorus Determination Method
08:21

Optimized Procedure for Determining the Adsorption of Phosphonates onto Granular Ferric Hydroxide using a Miniaturized Phosphorus Determination Method

Published on: May 18, 2018

15.0K
Bergmeyer Glucose Quantification for Microbiological Samples
07:23

Bergmeyer Glucose Quantification for Microbiological Samples

Published on: January 17, 2025

991

Related Experiment Videos

Last Updated: Dec 24, 2025

Measuring Phagosome pH by Ratiometric Fluorescence Microscopy
14:39

Measuring Phagosome pH by Ratiometric Fluorescence Microscopy

Published on: December 7, 2015

14.3K
Optimized Procedure for Determining the Adsorption of Phosphonates onto Granular Ferric Hydroxide using a Miniaturized Phosphorus Determination Method
08:21

Optimized Procedure for Determining the Adsorption of Phosphonates onto Granular Ferric Hydroxide using a Miniaturized Phosphorus Determination Method

Published on: May 18, 2018

15.0K
Bergmeyer Glucose Quantification for Microbiological Samples
07:23

Bergmeyer Glucose Quantification for Microbiological Samples

Published on: January 17, 2025

991

Area of Science:

  • Analytical Chemistry
  • Physical Chemistry

Background:

  • pH indicators are versatile sensors for monitoring pH changes in diverse environments.
  • Mixtures of indicators are essential for broad pH range measurements, while single indicators suit narrow ranges.

Purpose of the Study:

  • To present a novel methodology for precise pH determination using indicator mixtures.
  • To validate the method for wide pH ranges and dynamic chemical reactions.

Main Methods:

  • Utilized fundamental laws of mass action and mass conservation for pH calculations.
  • Employed mixtures of neutral red, phenol red, and methyl orange indicators.
  • Applied the method to static solutions and stopped-flow kinetic measurements.

Main Results:

  • Successfully determined pH values for solutions across a broad range (0.5-9).
  • Demonstrated the method's efficacy in rapid pH monitoring of CO2 reactions in aqueous amine solutions.

Conclusions:

  • The proposed methodology provides accurate pH determination using indicator mixtures.
  • This approach is suitable for both equilibrium and kinetic pH measurements.