The effect of hyperbaric oxygen on mitochondrial and glycolytic energy metabolism: the caloristasis concept
Related Experiment Video
Updated: Dec 24, 2025

An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes
Published on: November 21, 2016
Didem Tezgin1, Charles Giardina1, George A Perdrizet2
1Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT, 06269-3125, USA.
Abstract:
We present new data on the effects of HBOT on human kidney (HK-2) cell metabolism using a SeaHorse XF Analyzer to evaluate separately the state of mitochondrial and glycolytic energy metabolism. The data are discussed in the context of the concept of cellular caloristasis networks. The information on the changes in cellular energy metabolism stimulated by HBOT presented here provides new insights into the cellular energy state and mitochondrial environment in which sHSPs function. These data will be useful in forming testable hypotheses about the functions of translocated sHSPs in human mitochondria responding to stressors.
Related Concept Videos
Oxygen Transport in the Blood
Muscle Recovery and Fatigue
Chemiosmosis
Electron Transport Chain
The electron transport chain involves a series of protein complexes on the inner mitochondrial membrane that undergo a series of redox reactions. At the end of this chain, the electrons...
Outcomes of Glycolysis
Cellular respiration can occur aerobically (with oxygen) or anaerobically (without oxygen). In the presence of oxygen, cellular respiration starts with glycolysis and continues with pyruvate...
Metabolic Rate
The Basal Metabolic Rate (BMR) measures the energy expended at rest.
Several factors influence...
Electron Transport Chain: Complex I and II
ROS generation is regulated and maintained at moderate levels necessary...

