Related Experiment Video
Updated: Dec 24, 2025
09:29
Mycobacterium tuberculosis Extracellular Vesicle Enrichment through Size Exclusion Chromatography
Published on: May 19, 2022
2.7K
Rv0100, a proposed acyl carrier protein in Mycobacterium tuberculosis: expression, purification and crystallization.
Jasper Marc G Bondoc1, Hiten J Gutka1, Mashal M Almutairi1
1Institute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, 833 South Wood Street, Chicago, IL 60612, USA.
Summary
The protein identity within Bondoc et al.
Area of Science:
- Structural biology
- Protein crystallography
Background:
- Crystallographic studies are essential for determining protein structures.
- Accurate protein identification is crucial for interpreting structural data.
Purpose of the Study:
- To clarify the precise protein identity in crystals previously reported.
- To correct potential misidentification in prior crystallographic studies.
Main Methods:
- Re-analysis of crystallographic data.
- Comparison with known protein sequences and structures.
Main Results:
- The protein in the reported crystals is definitively identified.
- The identity aligns with specific protein families based on structural and sequence homology.
Conclusions:
- This study resolves ambiguity regarding the protein's identity.
- Ensures accurate foundation for future structural and functional studies.
