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Exploring the complex map of insulin polymorphism: a novel crystalline form in the presence of m-cresol
Fotini Karavassili1, Alexandros Valmas1, Maria Dimarogona2
1Department of Biology, University of Patras, 26500 Patras, Greece.
Abstract:
In this study, the first crystal structure of a novel crystal form of human insulin bound to meta-cresol in an acidic environment is reported. The combination of single-crystal and powder X-ray diffraction crystallography led to the detection of a previously unknown monoclinic phase (P21). The structure was identified from the powder patterns and was solved using single-crystal diffraction data at 2.2 Å resolution. The unit-cell parameters at pH 6.1 are a = 47.66, b = 70.36, c = 84.75 Å, β = 105.21°. The structure consists of two insulin hexamers per asymmetric unit. The potential use of this insulin form in microcrystalline drugs is discussed.
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