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Multiple microarrays of non-adherent cells on a single 3D stimuli-responsive binary polymer-brush pattern
Jianwen Hou1, Runhai Chen, Jingchuan Liu
1State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China. shiqiang@ciac.ac.cn.
Journal of Materials Chemistry. B
|April 8, 2020
Summary
Researchers created a smart polymer pattern to precisely arrange multiple cell microarrays using temperature and lectins. This technique enables specific cell capture for advanced bioassays and sensors.
Area of Science:
- Materials Science
- Biotechnology
- Surface Chemistry
Background:
- Developing advanced platforms for precise cell manipulation is crucial for bioassays and diagnostics.
- Polymer brushes offer tunable surface properties for biomolecule and cell interactions.
Purpose of the Study:
- To engineer a 3D smart binary polymer-brush pattern for creating multiple cell microarrays.
- To utilize thermo-responsive and specific binding properties for site-specific cell capture.
Main Methods:
- Fabrication of binary polymer brushes using photolithography and surface-initiated photo-polymerization (SIPP).
- Utilizing poly(N-isopropylacrylamide) (PNIPAM) for thermo-responsive red blood cell (RBC) adhesion.
- Employing poly(d-gluconamidoethyl methacrylate) (PGAMA) brushes for concanavalin A (Con A) mediated site-specific RBC capture.
Main Results:
- Successfully fabricated well-defined, high-resolution binary polymer-brush patterns.
- Demonstrated thermo-reversible switching of PNIPAM brushes for controlled RBC adhesion.
- Achieved specific RBC capture via Con A binding to PGAMA domains, creating multiple cell microarrays on a single platform.
Conclusions:
- A novel, versatile, and specific platform for generating multiple cell microarrays was developed.
- The smart binary polymer-brush pattern shows potential for applications in microsensors, biochips, and bioassays.
- This method offers a facile approach for advanced cell microarray fabrication.
