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Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Updated: Dec 24, 2025

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

Multiplexed fluorescence lifetime imaging by concentration-dependent quenching.

Teng Luo¹, Ting Zhou, Yihua Zhao

¹Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China. liulw@szu.edu.cn jlqu@szu.edu.cn.

Journal of Materials Chemistry. B

|April 8, 2020

Summary

This summary is machine-generated.

This study introduces a simple multiplexed imaging analysis using eosin fluorescence lifetimes for histopathological tissue identification. This method distinguishes tissues based on eosin concentration-dependent quenching, simplifying pathological diagnosis.

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Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

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Published on: January 18, 2017

Area of Science:

Biomedical Optics
Histopathology
Fluorescence Microscopy

Background:

Multiplexed imaging in histopathology often requires complex techniques.
Concentration-dependent quenching of fluorescent dyes can be an overlooked phenomenon.
Hematoxylin and eosin (H&E) staining is a standard in tissue analysis.

Purpose of the Study:

To develop a simplified multiplexed imaging analysis for histopathological identification.
To leverage eosin's concentration-dependent fluorescence quenching for tissue differentiation.
To explore the potential of fluorescence lifetime imaging microscopy (FLIM) in pathological diagnosis.

Main Methods:

Investigated the relationship between eosin concentration and fluorescence lifetime.
Utilized fluorescence lifetime imaging microscopy (FLIM) on H&E stained tissue sections.
Analyzed average fluorescence lifetime histograms for tissue separation.

Main Results:

Eosin fluorescence lifetime exhibits concentration-dependent quenching and recovery patterns.
Multiplexed imaging was achieved in situ using only eosin fluorescence lifetimes.
Different H&E stained tissue components (erythrocytes, muscle, collagen) were identified based on eosin concentration.

Conclusions:

Eosin fluorescence lifetimes can simplify multiplexed imaging in histopathology.
The method allows for tissue differentiation without complex staining or excitation techniques.
This approach shows potential for improving pathological diagnostic capabilities.