Eu/Tb luminescence for alkaline phosphatase and β-galactosidase assay in hydrogels and on paper devices

Simple technologies for efficient detection of important (bio)molecules are always in great demand. We now report the detection and assay of two biologically important enzymes, alkaline phosphatase and β-galactosidase, in Eu- or Tb-based cholate hydrogels, respectively, and on filter paper discs coated with such hydrogels. Pro-sensitizers derived from 1-hydroxypyrene and 2,3-dihydroxynaphthalene were incorporated into Eu or Tb cholate hydrogels, respectively. Upon enzyme action, these artificial substrates liberate free sensitizers both in the gel and on gel-coated discs, resulting in turn-on luminescence, red/magenta for Eu, and green for Tb. The detection of enzymes was also demonstrated in natural/biological samples using low-cost systems.