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Updated: Dec 24, 2025

Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator
Published on: October 20, 2021
A redox-responsive folate-fluorophore conjugate as a new target-cell-specific imaging probe
Jisu Kim1, Hyunjin Kim, Yongdoo Choi
1Biomarker Branch, National Cancer Center, 323 Ilsan-ro, Goyang-si, Gyeonggi-do 10408, Republic of Korea. ydchoi@ncc.re.kr.
Abstract:
Here we have developed a redox-responsive folate-fluorophore conjugate (RFC) with a disulfide linker for the target-specific imaging of cancers. The quenched fluorescence of the RFC could be turned on after folate receptor-mediated endocytosis and subsequent cleavage of the disulfide linker by intracellular glutathione, enabling target-cell-specific fluorescence imaging with a high target-to-background ratio.
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