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Microfluidic channels with renewable and switchable biological functionalities based on host-guest interactions.

Siyuan Li1, Bing Liu, Ting Wei

  • 1State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, 215123, P. R. China. liuxiaoli@suda.edu.cn chenh@suda.edu.cn.

Journal of Materials Chemistry. B
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Summary

Researchers developed a new method to modify poly(dimethylsiloxane) (PDMS) microfluidic channels. This technique creates surfaces that can be renewed and switched for detecting specific proteins and bacteria.

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Area of Science:

  • Materials Science
  • Biotechnology
  • Chemical Engineering

Background:

  • Poly(dimethylsiloxane) (PDMS) is widely used in microfluidics due to its favorable properties.
  • However, PDMS's hydrophobicity and chemical inertness limit its applications.
  • Surface modification is crucial for enhancing PDMS microfluidic channel functionality.

Purpose of the Study:

  • To develop a novel surface modification strategy for PDMS microfluidic channels.
  • To create PDMS surfaces with renewable and switchable biofunctions.
  • To enable specific detection and measurement of proteins and bacteria.

Main Methods:

  • Graft copolymerization of oligo(ethylene glycol)methacrylate (OEGMA) and OEGMA-adamantane (OEGMA-Ada) onto PDMS surfaces via photochemistry.
  • Attachment of biotin or mannose-decorated beta-cyclodextrin (β-CD) using host-guest interactions.
  • Demonstration of renewable and switchable biofunctions using SDS treatment.

Main Results:

  • Successfully created modified PDMS microchannels (PDMS-POA) with OEGMA and OEGMA-Ada.
  • Achieved specific binding of biotin and bacterial adhesion via β-CD derivatives.
  • Demonstrated that the biofunctions of the microchannels were renewable and switchable.

Conclusions:

  • The combination of photochemical grafting and host-guest chemistry offers a versatile method for PDMS surface modification.
  • This approach yields microfluidic channels with tunable and reversible biofunctions.
  • The developed PDMS-POA microchannels are suitable for sensitive detection and measurement of specific biomolecules and microorganisms.