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Related Concept Videos

Nucleic acids02:43

Nucleic acids

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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
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Polymers and hydrogels for local nucleic acid delivery.

Lies A L Fliervoet1, Johan F J Engbersen, Raymond M Schiffelers

  • 1Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, PO Box 80082, 3508 TB Utrecht, The Netherlands. t.vermonden@uu.nl.

Journal of Materials Chemistry. B
|April 8, 2020
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Summary

Local gene therapy using hydrogels offers a promising approach to treat diseases by improving nucleic acid delivery. This review explores the design of polymeric hydrogels for enhanced gene therapy applications.

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Area of Science:

  • Biomaterials Science
  • Gene Therapy
  • Drug Delivery

Background:

  • Gene therapy holds significant potential for treating chronic and life-threatening diseases.
  • Delivery of nucleic acids to intracellular targets remains a major challenge for widespread gene therapy application.
  • Local delivery strategies, particularly using hydrogels, are emerging to overcome these challenges.

Purpose of the Study:

  • To review the rational design of polymeric and hydrogel materials for local gene therapy.
  • To highlight how hydrogel-based systems can enhance in vivo efficacy and limit off-target effects.
  • To discuss the critical design features for efficient nucleic acid delivery via hydrogels.

Main Methods:

  • Review of existing literature on hydrogel-based nucleic acid delivery systems.
  • Analysis of polymer selection for hydrogel and polyplex particle formation.
  • Discussion of entrapment methods (conjugates, polyplexes) for controlled release.

Main Results:

  • Hydrogels enable local and controlled release of nucleic acids, improving therapeutic outcomes.
  • Careful selection of polymers is crucial for both particle and hydrogel fabrication.
  • Entrapment of nucleic acids as conjugates or polyplexes within hydrogels facilitates efficient delivery.

Conclusions:

  • Polymeric hydrogels represent a viable strategy for localized gene therapy.
  • Rational design of these materials is key to overcoming current gene delivery limitations.
  • Further research into material design will advance the clinical application of gene therapy.