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Updated: Dec 24, 2025
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
Published on: December 9, 2013
Simultaneous near-infrared and green fluorescence from single conjugated polymer dots with aggregation-induced
Sitong Chen1, Shuang Cui, Rongxin Du
1School of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, P. R. China. yongzhang@hit.edu.cn.
Abstract:
Near-infrared fluorescent probes are important and interesting for advanced biological and biomedical applications where the fluorescence technology is employed. Herein, we reported high near-infrared (NIR) fluorescent conjugated polymer dots (Pdots) of two polymers (PTPE-DTNT2 and PTPE-DTNT4) with cross-conjugated aggregation-induced emission fluorogen as the side chain. The photoluminescent quantum yields (PLQYs) of Pdots were up to 31.8% with the near-infrared emission peak of 716 nm, which is among the best values of NIR Pdots reported so far. In addition, Pdots possessed a dual-color emission feature. Thus, dual-color cellular imaging with both Pdots was demonstrated in biological applications; to the best of our knowledge, this is the first reported dual-color cellular imaging with Pdots. The NIR Pdots were also applied in in vivo cytotoxicity analysis and microangiography imaging of zebrafish. We anticipate that NIR Pdots with their dual-color feature will find broad applications in multiplexed biological and biomedical fields.
