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Updated: Dec 24, 2025
A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
Published on: June 27, 2022
A novel FRET-based fluorescent probe for the selective detection of hydrogen sulfide (H2S) and its application for
Qi Zhao1, Fangjun Huo, Jin Kang
1Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Key Laboratory of Materials for Energy Conversion and Storage of Shanxi Province, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China. yincx@sxu.edu.cn.
Abstract:
Hydrogen sulfide (H2S) is an endogenously produced gaseous signaling molecule with multiple functions in many physiological and pathological processes. Herein, our group developed a novel fluorescent probe (Flu-N3) for H2S on the basis of the 7-amino-4-methylcoumarin and fluorescein FRET system. Adding H2S to a solution of Flu-N3 resulted in a clearly observable fluorescence enhancement (50-fold). This probe showed high sensitivity and selectivity towards H2S over other reactive species with a detection limit of 0.031 μM and a fast response time (within 8 s). Moreover, Flu-N3 displayed little toxicity to HepG-2 cells and was successfully applied to image exogenous and endogenous H2S in living cells and nude mice.
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