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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
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Updated: Dec 24, 2025

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Fluorescence Image-Guided Surgery - a Perspective on Contrast Agent Development.

Connor W Barth1, Summer L Gibbs1,2,3

  • 1Biomedical Engineering Department, Oregon Health & Science University, Portland, OR 97201.

Proceedings of Spie--The International Society for Optical Engineering
|April 8, 2020
PubMed
Summary
This summary is machine-generated.

Novel fluorescence image-guided surgery (FGS) contrast agents are advancing rapidly, with 39 agents in 85 trials. Four agents are in Phase III, nearing FDA approval, promising improved surgical outcomes.

Keywords:
clinical developmentclinical trialcontrast agentfluorescencefluorescence imaging systemimage-guided surgerynear-infrared fluorescence

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Area of Science:

  • Medical Imaging
  • Surgical Technology
  • Biomedical Engineering

Background:

  • Numerous novel fluorescence image-guided surgery (FGS) contrast agents have been developed over decades.
  • Many of these agents are currently in clinical translation and undergoing trials.

Purpose of the Study:

  • To review and summarize novel FGS contrast agents currently in clinical translation.
  • To assess the status of clinical trials and FDA approval pathways for these agents.

Main Methods:

  • Identification and summarization of FGS contrast agents in clinical translation.
  • Analysis of clinical trial data, including number of trials and phases.
  • Review of probe types, targeting mechanisms, and fluorescence properties.

Main Results:

  • 39 novel FGS contrast agents are under investigation in 85 clinical trials.
  • Four agents are in Phase III trials, with potential FDA approval in 2-3 years.
  • Significant diversity exists in probe types, targeting mechanisms, and fluorescence properties.

Conclusions:

  • Further development of FGS imaging systems is needed to match the variety of agents in development.
  • Expanded studies for additional disease types and anatomy-specific agents are required.
  • Continued development of FGS technology will significantly impact surgical outcomes across specialties.