Related Experiment Video

Updated: Dec 24, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.9K

miR-145-5p Regulates the Proliferation, Migration and Invasion in Cervical Carcinoma by Targeting KLF5

Hui Cao1, Guihua Pan1, Shiqiang Tang1

  • 1Department of Oncology, Chenzhou First People's Hospital, Chenzhou, People's Republic of China.

Oncotargets and Therapy
|April 8, 2020
PubMed
Abstract

Insights

MicroRNA-145-5p (miR-145-5p) is downregulated in cervical carcinoma (CC). Restoring miR-145-5p inhibits CC cell proliferation, migration, and invasion by targeting KLF5.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Cervical carcinoma (CC) poses a significant threat to women's health.
  • Limited effective therapeutic strategies are currently available for CC.
  • Understanding the molecular mechanisms underlying CC progression is crucial for developing novel treatments.

Purpose of the Study:

  • To investigate the role and mechanism of microRNA-145-5p (miR-145-5p) in cervical carcinoma (CC).
  • To explore the potential of miR-145-5p as a therapeutic target for CC.

Main Methods:

  • Bioinformatic analysis of CC gene expression data from TCGA.
  • qRT-PCR and Western blot to assess miR-145-5p and KLF5 expression.
  • Dual-luciferase reporter assay to confirm direct targeting.
  • Cell proliferation, migration, and invasion assays (CCK-8, wound healing, Transwell).

Main Results:

  • miR-145-5p was significantly downregulated in CC tissues and cells.
  • KLF5 expression was inversely correlated with miR-145-5p levels.
  • miR-145-5p directly targets KLF5, reducing its expression.
  • Overexpression of miR-145-5p or silencing of KLF5 inhibited CC cell proliferation, migration, and invasion.

Conclusions:

  • miR-145-5p acts as a tumor suppressor in cervical carcinoma.
  • The miR-145-5p/KLF5 axis is a key regulator of CC cell behavior.
  • Targeting miR-145-5p may offer a novel therapeutic strategy for cervical carcinoma.
Keywords:
KLF5cervical carcinomainvasionmiR-145-5pmigrationproliferation

More Related Videos

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells11:42

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Published on: April 7, 2017

9.8K
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.7K

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.0K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.6K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.8K
Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
3.1K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
5.2K
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.6K