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The single-grain method: adding TEM to the equation.

Silvia Ulrich1, Friđgeir Grímsson1

  • 1Department of Botany and Biodiversity Research, University of Vienna, Vienna, Austria.

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|April 8, 2020
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Summary
This summary is machine-generated.

A new protocol simplifies preparing single pollen grains for transmission electron microscopy (TEM), enabling faster analysis of pollen and spore ultrastructure.

Keywords:
fossil pollenlight microscopypollen morphologypollen ultrastructurescanning electron microscopytransmission electron microscopy

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Area of Science:

  • Paleontology
  • Microscopy
  • Palynology

Background:

  • Transmission electron microscopy (TEM) is crucial for studying pollen and spore ultrastructure.
  • Existing methods for preparing single pollen grains for TEM can be time-consuming and result in sample loss.

Purpose of the Study:

  • To develop an advanced, efficient protocol for preparing individual extant and fossil pollen grains for TEM analysis.
  • To improve upon existing single-grain preparation techniques for palynological research.

Main Methods:

  • A novel protocol for the meticulous preparation of single pollen grains.
  • Integration with existing light and scanning electron microscopy techniques for fossil pollen investigation.

Main Results:

  • The protocol facilitates rapid recovery of ultrastructural data from single pollen and spores.
  • It effectively prevents the loss of pollen grains and ensures successful preparation of even difficult-to-locate specimens.
  • The method is user-friendly and significantly reduces preparation time compared to prior techniques.

Conclusions:

  • This advanced protocol offers a significant improvement for TEM-based palynological studies.
  • It enhances the efficiency and success rate of analyzing pollen and spore ultrastructure, complementing established methods.