Related Experiment Video
Updated: Dec 24, 2025
Stem Cell-Derived Viral Ag-Specific T Lymphocytes Suppress HBV Replication in Mice
Published on: September 25, 2019
Increased Expression of Myeloid-Derived Suppressor Cells in Patients with HBV-Related Hepatocellular Carcinoma
Tianyu Li1, Xinyu Zhang1, Zhuo Lv1
1Graduate College of Hebei Medical University, Hebei Medical University, Shijiazhuang, Hebei 050017, China.
Insights
Myeloid-derived suppressor cells (MDSCs) are elevated in hepatocellular carcinoma (HCC) and may drive disease progression. Inhibiting MDSCs early could be a therapeutic strategy for HCC.
Area of Science:
- Immunology
- Hepatology
- Oncology
Background:
- Hepatocellular carcinoma (HCC) is a major global health concern, often developing from chronic hepatitis B (CHB) infection.
- Myeloid-derived suppressor cells (MDSCs) are known to play a role in immune suppression and tumor progression.
Purpose of the Study:
- To investigate the role of MDSCs in the progression of chronic hepatitis B to HCC.
- To assess the correlation of MDSCs with clinical parameters and immune cell function in HCC patients.
Main Methods:
- Flow cytometry was used to quantify MDSCs and IFN-γ-producing T cells in peripheral blood.
- ELISA was employed to measure serum IL-10 and TNF-α levels.
- Statistical analyses were performed to determine associations between MDSCs and various clinical and immunological markers.
Main Results:
- MDSC percentages, particularly PMN-MDSCs, were significantly higher in HCC patients compared to CHB patients and healthy controls.
- MDSC levels correlated with indicators of liver function, systemic inflammation, and monocyte counts.
- HCC patients exhibited reduced IFN-γ-producing CD8 T cells, but no direct relationship was found between MDSCs and these cells. IL-10 levels were elevated in HCC patients.
Conclusions:
- MDSCs are implicated in the pathogenesis of HCC development from chronic HBV infection.
- Targeting MDSCs early in the disease process may represent a potential therapeutic approach to impede HCC progression.
Methods:
The percentages of MDSCs, IFN-γ-producing CD4 and CD8 T cells in the peripheral blood of HCC patients, chronic hepatitis B (CHB) patients, and healthy controls (HC) were determined by flow cytometry. The serum concentrations of IL-10 and TNF-α were determined by ELISA. The association of the percentages of MDSCs with tumor burden, liver function parameters, systemic inflammation-related indexes, and IFN-γ-producing T cells was assessed.
Results:
The percentages of MDSCs and PMN-MDSCs were significantly higher in HCC patients than those in CHB patients and HC. The level of MDSCs was correlated with indirect bilirubin and prealbumin, as well as systemic inflammation response index, monocyte/lymphocyte ratio, and monocyte counts. The frequency of IFN-γ-producing CD8 T cells of HCC patients was lower than that of HC. However, there was no relationship between MDSCs and IFN-γ-producing CD8 T cells. The level of IL-10 in HCC patients was significantly higher than that in CHB patients.
Conclusion:
MDSCs seem to play an important role in the process leading from chronic HBV infection to HCC. Early inhibiting these cells could affect tumor progression.
More Related Videos
14:15Preparation of Myeloid Derived Suppressor Cells MDSC from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting AutoMACS
Published on: June 18, 2012
05:55Identifying Inhibitors of the HBx-DDB1 Interaction Using a Split Luciferase Assay System
Published on: December 21, 2019
Related Concept Videos
01:01Regulation of Hematopoietic Stem Cells
02:23Abnormal Proliferation
01:19Multipotency of Hematopoietic Stem Cells