Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Multi-omics analysis of extracellular vesicle cargo in cancer.

Trends in cancer·2026

Same author

DiaReport: Reproducible workflow for differential expression analysis and interactive reporting in DIA-based proteomics.

Bioinformatics (Oxford, England)·2026

Same author

A living biobank of sarcoma patient-derived cell cultures reveals multi-omic and functional insights that capture disease heterogeneity.

Clinical and translational medicine·2026

Same author

A Combined Omics Approach to Elucidate the Molecular Interplay behind a Beneficial <i>Arabidopsis-Caulobacter</i> Interaction.

Journal of proteome research·2026

Same author

Proteomic profiling of whole tissue sections in cardiac ATTR amyloidosis reveals increased extracellular matrix remodeling.

Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology·2026

Same author

Pneumococcus uses COMMD2 to alter host cellular immunity.

Nature communications·2026

Same journal

NMR Spectroscopy: Molecular Insights into Cell Wall Collapse and Oxidative Stress of <i>Escherichia coli</i> Induced by Imidazole-Activated Eutectic Solvents.

ACS omega·2026

Same journal

Enhanced Arsenite Remediation in Synthetic FeS<sub>2</sub>/Fe(II)-Containing Arsenic Wastewater via Epigallocatechin Gallate-Initiated Persulfate Activation.

ACS omega·2026

Same journal

Defect and Particle-Size Engineering as Mechanistic Drivers for Dye Uptake in a Zirconium Metal-Organic Framework.

ACS omega·2026

Same journal

Biogeochemical Assessment of Short-Term Hydrogen Storage in Methane Reservoirs with Field Sample Characterization and Reactor Experiments.

ACS omega·2026

Same journal

Combined Effects of Halloysite Nanotubes, Nucleating Agent, and Thermal Annealing on the Printability and Mechanical Performances of 3D-Printable Polypropylene Random Copolymer-Based Composites.

ACS omega·2026

Same journal

Effect of MoS<sub>2</sub> Interfacial Engineering across MAPbI<sub>3</sub>, FAPbI<sub>3</sub>, and CsPbI<sub>3</sub> Perovskite Solar Cells.

ACS omega·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Dec 24, 2025

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling

Published on: July 1, 2014

Simple Peptide Quantification Approach for MS-Based Proteomics Quality Control.

Teresa Mendes Maia^1,2,3, An Staes^1,2,3, Kim Plasman⁴

¹VIB Center for Medical Biotechnology, Albert Baertsoenkaai 3, Ghent 9000, Belgium.

|April 8, 2020

Summary

This summary is machine-generated.

Quantifying peptides after digestion is crucial for bottom-up proteomics quality control. A microfluidic UV/Vis spectrophotometer enables accurate peptide quantification, improving LC-MS/MS and protein quantification data variability.

More Related Videos

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Published on: March 23, 2020

Related Experiment Videos

Last Updated: Dec 24, 2025

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling

Published on: July 1, 2014

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Published on: March 23, 2020

Area of Science:

Proteomics
Analytical Chemistry
Biotechnology

Background:

Bottom-up proteomics is a complex workflow involving sample preparation, LC-MS/MS, and data analysis.
Quality assessment is vital but lacks routine methods for quantifying peptide losses post-digestion.

Purpose of the Study:

To introduce a microfluidic UV/Vis spectrophotometer for accurate MS-ready peptide quantification.
To determine optimal sample loading for LC-MS/MS and assess its impact on data variability.

Main Methods:

Utilized a microfluidic UV/Vis spectrophotometer for direct peptide quantification in MS-loading solvent (2 μL sample).
Compared microfluidic spectrophotometer with a standard device.
Determined optimal peptide amount (3 μg) for LC-MS/MS on a Q Exactive HF mass spectrometer using K562 cell digest dilution series.
Analyzed tryptic digests from HEK293T cells to evaluate data variability.

Main Results:

The microfluidic spectrophotometer offers accurate and rapid peptide quantification.
Established 3 μg as the optimal peptide injection amount for the specified LC-MS/MS system.
Injecting equal peptide amounts significantly reduces variability in LC-MS/MS and protein quantification.

Conclusions:

Microfluidic UV/Vis spectrophotometry provides an easy and accurate method for routine peptide quantification in proteomics.
This approach enhances quality control by minimizing sample loss and improving data consistency.
Routine peptide quantification is a feasible next step for daily proteomics quality control.