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Related Concept Videos

Open Angle Glaucoma: Treatment01:27

Open Angle Glaucoma: Treatment

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In open-angle glaucoma, the iridocorneal angle remains open, but the trabecular meshwork becomes stiff, slowing down the outflow of aqueous humor. This causes a buildup of aqueous humor in the anterior chamber, leading to a sudden increase in intraocular pressure. The treatment for open-angle glaucoma focuses on reducing the elevated intraocular pressure by either decreasing the secretion of aqueous humor or increasing its outflow.
Drugs such as carbonic anhydrase inhibitors, α2- and...
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Glaucoma: Overview01:25

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Glaucoma is an eye condition characterized by increased intraocular pressure that damages the retina and optic nerve, leading to irreversible blindness if left untreated. The human eye has various components, including the cornea, iris, pupil, lens, and optic nerve. Aqueous humor is secreted by the epithelium of the ciliary body in the posterior chamber and flows through the trabecular meshwork and canal of Schlemm, maintaining normal intraocular pressure. The trabecular meshwork and the canal...
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Related Experiment Video

Updated: Dec 24, 2025

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye
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Adipose-derived stem cells integrate into trabecular meshwork with glaucoma treatment potential.

Yi Zhou1,2, Xiaobo Xia2, Enzhi Yang1

  • 1Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA, USA.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|April 8, 2020
PubMed
Summary

Human adipose-derived stem cells (ADSCs) can be differentiated into trabecular meshwork (TM) cells. Transplanted ADSC-TM cells restored TM structure and function, normalizing intraocular pressure (IOP) in mouse models, offering a potential glaucoma treatment.

Keywords:
adipose stem cells; differentiationhomingintegrationtrabecular meshwork

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Area of Science:

  • Ophthalmology
  • Regenerative Medicine
  • Cell Biology

Background:

  • The trabecular meshwork (TM) is crucial for maintaining intraocular pressure (IOP).
  • Glaucoma is characterized by reduced TM cellularity and often elevated IOP.
  • Current treatments for glaucoma aim to lower IOP but do not restore TM tissue.

Purpose of the Study:

  • To investigate the potential of human adipose-derived stem cells (ADSCs) for TM regeneration.
  • To induce ADSCs into TM cells (ADSC-TM) in vitro and assess their characteristics.
  • To evaluate the efficacy of ADSC-TM cell transplantation in restoring TM function and IOP in vivo.

Main Methods:

  • ADSCs were differentiated into TM cells in vitro.
  • ADSC-TM cells were characterized for genotypic profile, phagocytic activity, and response to dexamethasone.
  • ADSCs and ADSC-TM cells were transplanted into naive mouse eyes.
  • Post-transplantation, TM tissue integration, cell marker expression, IOP, outflow facility, and extracellular matrix were assessed.
  • Cell migration and homing mechanisms, specifically the CXCR4/SDF1 pathway, were investigated.

Main Results:

  • ADSC-TM cells exhibited a TM cell-like genotypic profile and functional characteristics.
  • Transplanted ADSCs and ADSC-TM cells successfully integrated into the mouse TM tissue.
  • Integrated cells expressed TM cell markers and maintained normal IOP, outflow facility, and extracellular matrix.
  • The chemokine pair CXCR4/SDF1 was identified as potentially important for ADSC-TM cell homing.

Conclusions:

  • Human ADSCs can be differentiated into functional TM cells.
  • ADSC-based therapies hold promise for restoring TM structure and function in glaucoma.
  • This approach offers a potential strategy for normalizing IOP and treating glaucoma using autologous or allogeneic stem cells.