Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Vaccinations01:51

Vaccinations

50.9K
Overview
50.9K
Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

845
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
845
Cancer Vaccines01:30

Cancer Vaccines

872
Cancer treatment vaccines are a rapidly evolving field that offers a promising approach to immunotherapy. Unlike traditional vaccines that prevent diseases, cancer treatment vaccines are designed to treat existing cancers by stimulating the immune system to recognize and attack cancer cells.
Cancer vaccines come in two categories: preventive (prophylactic) and treatment (active). Preventive vaccines, such as the Human Papillomavirus (HPV) vaccine, protect against viruses that cause certain...
872
Cross-reactivity00:42

Cross-reactivity

32.7K
Overview
32.7K
Active versus Passive Immunity01:31

Active versus Passive Immunity

9.7K
Immunity, along with the ability to limit pathogen growth to prevent significant body tissue damage, can be gained either by (1) actively developing an immune response within the individual after exposure to a pathogen or after getting vaccinated or (2) passively transferring immune components from an immune individual to one who is nonimmune. Both these forms of immunity can be found naturally and in medical practices.
Active Immunity
Active immunity refers to the resistance one develops...
9.7K
Immunological Memory01:23

Immunological Memory

14.7K
Immunological memory, a pivotal pillar of the adaptive immune system, is responsible for the body's ability to remember and respond more swiftly and effectively to previously encountered pathogens. This remarkable feature is what makes vaccines so effective in preventing diseases.
What is Immunological Memory?
Immunological memory is an integral function of the immune system that allows it to recognize and react more rapidly and effectively to pathogens previously encountered. This feature...
14.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multivalent DNA vaccines induce potent cellular responses and prolong BCG-mediated control of Mycobacterium tuberculosis.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

Engineering single-dose plasmid DNA for sustained in vivo delivery of designer incretins.

Trends in biotechnology·2026
Same author

Efficient in vivo assembly of DNA encoded polyvalent BTEs for dual antigen targeting for broadening therapeutic impact in ovarian cancer.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

Deep mining of the human antibody repertoire identifies frequent and genetically diverse CDRH3 topologies targetable by vaccination.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Editorial: New insights in nucleic acid approaches for vaccine and biologic delivery.

Frontiers in immunology·2026
Same author

Conformational landscape of HIV-1 Env from closed to fully open.

Nature communications·2026

Related Experiment Video

Updated: Dec 24, 2025

Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes
08:27

Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes

Published on: August 13, 2021

4.9K

DNA vaccines: prime time is now.

Ebony N Gary1, David B Weiner1

  • 1The Wistar Institute, Philadelphia, PA, United States.

Current Opinion in Immunology
|April 8, 2020
PubMed
Summary

Newer synthetic DNA vaccines show strong immune responses and good tolerability in clinical studies. Advanced delivery methods like electroporation significantly enhance vaccine effectiveness for infectious diseases.

Area of Science:

  • Biotechnology
  • Immunology
  • Vaccinology

Background:

  • Synthetic DNA vaccines have advanced rapidly, showing high immune potency and tolerability.
  • Traditional needle/syringe delivery is being surpassed by methods like jet delivery, gene gun, and electroporation (EP).

Purpose of the Study:

  • To review recent clinical studies of synthetic DNA vaccines for infectious diseases.
  • To highlight the role of adaptive electroporation in enhancing vaccine efficacy.
  • To summarize preclinical advancements in DNA-encoded biologics production.

Main Methods:

  • Focus on clinical trials utilizing synthetic DNA vaccines.
  • Emphasis on adaptive electroporation (EP) as a key delivery technology.
  • Review of advancements in DNA insert design and formulation.

More Related Videos

Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles
10:16

Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles

Published on: August 17, 2022

3.7K
Skin Tattooing As A Novel Approach For DNA Vaccine Delivery
06:37

Skin Tattooing As A Novel Approach For DNA Vaccine Delivery

Published on: October 18, 2012

16.5K

Related Experiment Videos

Last Updated: Dec 24, 2025

Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes
08:27

Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes

Published on: August 13, 2021

4.9K
Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles
10:16

Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles

Published on: August 17, 2022

3.7K
Skin Tattooing As A Novel Approach For DNA Vaccine Delivery
06:37

Skin Tattooing As A Novel Approach For DNA Vaccine Delivery

Published on: October 18, 2012

16.5K

Main Results:

  • Synthetic DNA vaccines demonstrate robust humoral and cellular immunity.
  • Advanced delivery methods, particularly EP, significantly improve immune responses.
  • Optimized DNA constructs and delivery enhance vaccine performance.

Conclusions:

  • Synthetic DNA vaccines, especially with advanced delivery like EP, are effective for infectious disease prevention and treatment.
  • These advances pave the way for in vivo production of synthetic DNA biologics.
  • Further research is ongoing for novel DNA-encoded therapeutics.