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Updated: Dec 24, 2025
Sample Preparation for Single Virion Atomic Force Microscopy and Super-resolution Fluorescence Imaging
Published on: January 2, 2014
Immobilization of highly oriented filamentous viruses onto polymer substrates
Toshiki Sawada1, Takeshi Serizawa
1Department of Organic and Polymeric Materials, Tokyo Institute of Technology, 2-12-1-H121 Ookayama, Meguro-ku, Tokyo 152-8550, Japan. serizawa@polymer.titech.ac.jp.
Abstract:
A simple dipping/sweeping-up process for the polymer substrates successfully immobilized genetically engineered filamentous viruses (M13 phages) in a highly orientated manner. The specific interaction between the peptides displayed on the phage terminus and the polymer species was essential for their orientation. The orientation manner was dependent on the sweeping-up speed and the phage concentration.
