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Updated: Dec 24, 2025
Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
Published on: November 10, 2016
Bifunctional quantum dot-decorated Ag@SiO2 nanostructures for simultaneous immunoassays of surface-enhanced Raman
Xianfeng Zhang1, Xianming Kong, Zhongpeng Lv
1Key Laboratory of Mesoscopic Chemistry (Ministry of Education), State Key Laboratory of Coordination Chemistry, and School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China. xzdu@nju.edu.cn.
Abstract:
Bifunctional nanostructured ensembles of quantum dot (QD)-decorated Ag@SiO2 nanoparticles embedded with Raman reporters p-aminothiophenol (PATP) were intentionally prepared for simultaneous immunoassays of surface-enhanced Raman scattering (SERS) and surface-enhanced fluorescence (SEF). The maximum SEF intensity was optimized with a silica shell spacer of about 9 nm. The SERS sensitivity was significantly improved due to the large Raman scattering cross-section of the coupling reaction product of PATP, generated on the Ag cores upon irradiation of laser during the SERS measurements. The antibody-immobilized QD-decorated Ag/PATP@SiO2 nanocomposites were used for antigen immunoassays using SERS and SEF with high sensitivity. This progress demonstrates the crucial role of rational design/control of multifunctional nanostructures in biodetection and bioimaging.
