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Researchers developed a novel, low-cost material by light-promoting biotinylation of SU-8 photoresist for effective biosensing applications. This method integrates photobiotin into the SU-8 curing process, reducing time and cost for spatial biosensor fabrication.

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Area of Science:

  • Materials Science
  • Biotechnology
  • Chemical Engineering

Background:

  • SU-8 photoresist is widely used in semiconductor, photonics, and lab-on-a-chip devices.
  • SU-8 is emerging as a promising alternative to silicon for sensing applications.
  • Immobilization of bioreceptors on surfaces is crucial for biosensor development.

Purpose of the Study:

  • To report light-promoted biotinylation of SU-8 polymer surfaces.
  • To develop a novel, effective, and low-cost material for bioreceptor immobilization and biosensing.
  • To demonstrate spatially resolved biosensing capabilities on SU-8 substrates.

Main Methods:

  • Direct incorporation of photobiotin into the SU-8 curing process.
  • Light-induced surface modification of SU-8 photoresist.
  • Immunoanalytical detection of the synthetic steroid gestrinone.

Main Results:

  • A novel material for spatial biosensing was successfully developed.
  • The photobiotin incorporation into SU-8 curing reduced fabrication time and cost.
  • Excellent performance was achieved in the detection of gestrinone.
  • The transparent SU-8 substrate enabled naked-eye biodetection and simple instrumental quantification.

Conclusions:

  • Light-promoted biotinylation of SU-8 offers a cost-effective and efficient method for biosensor fabrication.
  • The developed material enables spatially selective immobilization of bioreceptors.
  • This approach presents significant advantages for developing advanced biosensing platforms.