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Updated: Dec 24, 2025

Preparation and In Vitro Characterization of Dendrimer-based Contrast Agents for Magnetic Resonance Imaging
Published on: December 4, 2016
Water-soluble gold nanoparticles (AuNP) functionalized with a gadolinium(iii) chelate via Michael addition for use as
Mark Milne1, Pierangelo Gobbo, Nevin McVicar
1Department of Chemistry, The University of Western Ontario, London, Ontario N6A 5B7, Canada. robert.hudson@uwo.ca mworkent@uwo.ca.
Abstract:
A contrast agent suitable for magnetic resonance imaging based on small, water soluble gold nanoparticles (AuNP) conjugated to over 50 Gd3+ chelators has been prepared by using an interfacial Michael addition in aqueous media. The resultant chelator-AuNP conjugates have been successfully characterised by 1H NMR spectroscopy, IR spectroscopy, ICP-OES, ζ-potential analysis, TEM and MRI. T1-weighted in vivo images of mouse kidney were obtained using the agent at 9.4 T. A preliminary in vivo experiment produced no ill effects and the clearance profile of the agent suggests it is suitable for animal testing at clinically relevant concentrations.
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