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Fluorescence biolabeling using methylated silica nanoparticles containing a lanthanide complex.

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New fluorescent silica nanoparticles offer improved optical biolabeling for Candida albicans cells. Surface functionalization enhances cellular uptake, enabling selective cell visualization without nuclear membrane penetration.

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Area of Science:

  • Nanotechnology
  • Biomedical Engineering
  • Materials Science

Background:

  • Optical biolabels are crucial for cellular imaging.
  • Candida albicans infections require effective diagnostic tools.
  • Silica nanoparticles offer versatile platforms for biomedical applications.

Purpose of the Study:

  • To develop novel fluorescent silica nanoparticles for optical biolabeling.
  • To functionalize nanoparticles for enhanced cellular uptake in Candida albicans.
  • To investigate selective cell labeling capabilities.

Main Methods:

  • Encapsulation of a lanthanide picolinate complex (Tbpic) within silica particles via reverse microemulsion.
  • Surface functionalization of nanoparticles with 3-aminopropyltriethoxysilane.
  • Quaternization of nanoparticle surfaces with methyl iodide to improve water stability and cell uptake.
  • Confocal microscopy to assess nanoparticle uptake and localization in Candida albicans cells.

Main Results:

  • Successfully synthesized fluorescent core-shell silica nanoparticles containing a terbium(iii) picolinate complex.
  • Functionalized nanoparticles exhibited enhanced water stability and significantly improved uptake by Candida albicans compared to non-functionalized controls.
  • Confocal microscopy confirmed nanoparticle penetration of the cell wall but not the nuclear membrane, enabling selective cell biolabeling.

Conclusions:

  • Surface quaternization is an effective strategy for enhancing the cellular uptake of functionalized silica nanoparticles.
  • These novel fluorescent nanoparticles serve as promising optical biolabels for selective Candida albicans cell imaging.
  • The developed method offers a general approach for improving the biocompatibility and cellular targeting of nano-silica particles.