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Published on: March 16, 2017
Fluorene-derived two-photon fluorescent probes for specific and simultaneous bioimaging of endoplasmic reticulum and
Hua Zhang1, Jiangli Fan, Huijuan Dong
1State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, Hi-tech Zone, Dalian 116024, P.R. China. fanjl@dlut.edu.cn pengxj@dlut.edu.cn.
Abstract:
Two novel fluorene-derived two-photon fluorescent probes (TPFL) targeting the endoplasmic reticulum (TPFL-ER) and lysosomes (TPFL-Lyso) were synthesized by introducing a chlorine group and a morpholine group, respectively. They were shown to be suitable for specific and simultaneous imaging of the endoplasmic reticulum and lysosomes without affecting protein movements owing to their similar molecular structures. TPFL-ER and TPFL-Lyso were successfully used to visualize the changes of the endoplasmic reticulum and lysosomes during cancer cell apoptosis and they demonstrated high specificity and sensitivity, excellent photostability, and low phototoxicity.
