Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chirality in Nature02:30

Chirality in Nature

16.2K
Chirality is the most intriguing yet essential facet of nature, governing life’s biochemical processes and precision. It can be observed from a snail shell pattern in a macroscopic world to an amino acid, the minutest building block of life. Most of the snails around the world have right-coiled shells because of the intrinsic chirality in their genes. All the amino acids present in the human body exist in an enantiomerically pure state, except for glycine - the sole achiral amino acid.
16.2K
Chirality02:25

Chirality

28.7K
Chirality is a term that describes the lack of mirror symmetry in an object. In other words, chiral objects cannot be superposed on their mirror images. For example, our feet are chiral, as the mirror image of the left foot, the right foot, cannot be superposed on the left foot.
Chiral objects exhibit a sense of handedness when they interact with another chiral object. For example, our left foot can only fit in the left shoe and not in the right shoe. Achiral objects — objects that have...
28.7K
Prochirality02:05

Prochirality

4.7K
The concept of prochirality leads to the nomenclature of the individual faces of a molecule and plays a crucial role in the enantioselective reaction. It is a concept where two or more achiral molecules react to produce chiral products. A typical process is the reaction of an achiral ketone to generate a chiral alcohol. Here, the achiral reactant reacts with an achiral reducing agent, sodium borohydride, to generate an equimolar mixture of the chiral enantiomers of the product. For example, an...
4.7K
Molecules with Multiple Chiral Centers02:25

Molecules with Multiple Chiral Centers

14.6K
Molecules that possess multiple chiral centers can afford a large number of stereoisomers. For instance, while some molecules like 2-butanol have one chiral center, defined as a tetrahedral carbon atom with four different substituents attached, several molecules like butane-2,3-diol have multiple chiral centers. A simple formula to predict the number of stereoisomers possible for a molecule with n chiral centers is 2n. However, there can be a lower number where some of the stereoisomers are...
14.6K
Properties of Enantiomers and Optical Activity02:24

Properties of Enantiomers and Optical Activity

20.7K
It is essential to understand the difference between chiral and achiral interactions and the implications thereof in optical activity and their applications. Just as our feet, which are chiral, interact uniquely with chiral objects, such as a pair of shoes, but identically with achiral socks, enantiomers of a molecule exhibit different properties only when they interact with other chiral media. An example of a significant implication from this facet is the phenomenon known as optical activity,...
20.7K
Regioselectivity and Stereochemistry of Acid-Catalyzed Hydration02:34

Regioselectivity and Stereochemistry of Acid-Catalyzed Hydration

9.4K
The rate of acid-catalyzed hydration of alkenes depends on the alkene's structure, as the presence of alkyl substituents at the double bond can significantly influence the rate.
9.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessing Efgartigimod (VYVGART™) Social Media Posts in Generalized Myasthenia Gravis to Understand Patient, Caregiver, and Health Care Professional Sentiments.

Neurology and therapy·2026
Same author

The Conceptual Basis of Recurrence After Ligation of Intersphincteric Fistula Tract for Complex Anal Fistula.

Diseases of the colon and rectum·2026
Same author

Retraction notice to "Mobilization of lymphoblasts from bone marrow to peripheral blood in childhood acute lymphoblastic leukaemia: Role of 9-O-acetylated sialoglycoproteins" [Leukemia Res. 36(2) (2012) 146-155].

Leukemia research·2026
Same author

Retraction notice to"Nitric oxide sensing by chlorophyll a" [Anal. Chim. Acta 985 (2017) 101-113].

Analytica chimica acta·2026
Same author

Editorial Expression of Concern: Disease-associated glycosylated molecular variants of human C-reactive protein activate complement-mediated hemolysis of erythrocytes in tuberculosis and Indian visceral leishmaniasis.

Glycoconjugate journal·2026
Same author

Ultrasound-based scoring to predict long-term healing in anal fistula: A practical alternative or complementary to the MRI-based Garg scoring system?

Tropical doctor·2026

Related Experiment Video

Updated: Dec 16, 2025

A Micropatterning Assay for Measuring Cell Chirality
08:07

A Micropatterning Assay for Measuring Cell Chirality

Published on: March 11, 2022

2.6K

Cellular response to chirality and amplified chirality.

Sarita Roy1, Kaushik Bhattacharya, Chitra Mandal

  • 1Department of Biochemistry, University of Calcutta, 35 Ballygunge Circular Road, Kolkata-700019, India. adgcal@gmail.com.

Journal of Materials Chemistry. B
|April 9, 2020
PubMed
Summary

Cancer cells respond differently to chiral drug forms, penicillamine (PA), and their nano-conjugated versions. Amplified chirality via nano-conjugation alters cellular messages, impacting mitochondrial potential and caspase pathways.

More Related Videos

CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

7.4K
Coulomb Explosion Imaging as a Tool to Distinguish Between Stereoisomers
08:51

Coulomb Explosion Imaging as a Tool to Distinguish Between Stereoisomers

Published on: August 18, 2017

10.8K

Related Experiment Videos

Last Updated: Dec 16, 2025

A Micropatterning Assay for Measuring Cell Chirality
08:07

A Micropatterning Assay for Measuring Cell Chirality

Published on: March 11, 2022

2.6K
CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

7.4K
Coulomb Explosion Imaging as a Tool to Distinguish Between Stereoisomers
08:51

Coulomb Explosion Imaging as a Tool to Distinguish Between Stereoisomers

Published on: August 18, 2017

10.8K

Area of Science:

  • Biochemistry
  • Nanotechnology
  • Cancer Biology

Background:

  • Chirality plays a crucial role in biological systems.
  • Drug enantiomers can exhibit different pharmacological activities.
  • Nano-conjugation can amplify chiral properties.

Purpose of the Study:

  • To investigate the differential response of U87MG cancer cells to chiral forms of penicillamine (PA).
  • To explore the impact of nano-conjugation on cellular response to drug chirality.
  • To elucidate the mechanisms underlying cellular discrimination of chiral drug forms.

Main Methods:

  • Characterization of penicillamine enantiomers and silver nanoparticle conjugates using circular dichroism and fourier transform infrared spectroscopy.
  • Assessment of U87MG cancer cell response to chiral drug forms.
  • Analysis of mitochondrial membrane potential and caspase pathway activation.

Main Results:

  • U87MG cells exhibit distinct responses to l- and d-penicillamine.
  • Nano-conjugation of penicillamine with silver nanoparticles alters cellular responses.
  • Chirality and amplified chirality can induce dissimilar or reciprocal cellular effects.
  • Cellular discrimination involves modulation of mitochondrial membrane potential and caspase pathway elements.

Conclusions:

  • Cellular response to geometrical chirality is a complex, evolved mechanism.
  • Amplified chirality through nano-conjugation can lead to altered cellular signaling.
  • Understanding chiral drug interactions is vital for cancer therapy development.