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Highly cohesive dual nanoassemblies for complementary multiscale bioimaging.

Adrien Faucon1, Thomas Maldiney, Olivier Clément

  • 1CEISAM - UMR CNRS 6230, Université de Nantes, 2 rue de la Houssinière, 44322 Nantes, France. elena.ishow@univ-nantes.fr.

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|April 9, 2020
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Summary
This summary is machine-generated.

Researchers developed novel hybrid supraparticles combining magnetic nanoparticles and fluorescent molecules. These dual-function nanostructures enable high-resolution in vivo imaging and tracking of drug or stem cell delivery.

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Area of Science:

  • Nanotechnology
  • Biomedical Imaging
  • Materials Science

Background:

  • Developing advanced nanomaterials is crucial for sensitive biomedical diagnostics.
  • Simultaneous magnetic and fluorescent properties in nanostructures offer synergistic advantages for in vivo tracking.
  • Current imaging agents often lack the resolution or dual functionality needed for precise biodistribution studies.

Purpose of the Study:

  • To fabricate and characterize novel hybrid supraparticles with high payload of fluorophores and superparamagnetic nanoparticles.
  • To evaluate the in vivo performance of these dual nanoarchitectures for Magnetic Resonance Imaging (MRI) and fluorescence imaging.
  • To demonstrate the potential of these structures for high-resolution tracking of biodistribution.

Main Methods:

  • A two-step self-assembly process was employed to create core-shell hybrid supraparticles.
  • The supraparticles feature a fluorescent nanocore and a dense shell of superparamagnetic iron oxide nanoparticles.
  • In vivo MRI T2 contrast enhancement and two-photon excited fluorescence imaging were performed in small rodents.
  • Transmission Electron Microscopy (TEM) was used to image the nanoassemblies within sectioned mouse tissue.

Main Results:

  • The hybrid supraparticles exhibited enhanced NMR transverse relaxivity due to cooperative magnetic dipole interactions.
  • Significant in vivo MRI T2 contrast was achieved with dilute solutions after intravenous injection.
  • Unprecedented location resolution was obtained using two-photon excited fluorescence imaging.
  • The first TEM images of intact complex magnetic and fluorescent nanoassemblies in vivo were successfully acquired.

Conclusions:

  • The developed dual nanoarchitectures offer a powerful platform for high-resolution imaging.
  • These hybrid supraparticles demonstrate significant potential for assessing drug or labeled stem cell biodistribution.
  • The cohesive nature and dual functionality of these structures open new avenues in theranostics and biomedical research.