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Updated: Dec 24, 2025
Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry
Published on: July 17, 2019
Monitoring layer-by-layer self-assembly process of natural polyelectrolytes by fluorescent bioconjugate with
Jingwei Jia1, Zhengke Wang, Wentao Lu
1MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. wangzk@zju.edu.cn huql@zju.edu.cn.
Abstract:
A novel chitosan-based fluorescent bioconjugate (TPE-CS) with aggregation-induced emission (AIE) characteristic is synthesized and used as a fluorescent probe for monitoring layer-by-layer self-assembly process of natural polyelectrolytes. QCM results and contact angle measurement indicate that this AIE active TPE-CS bioconjugate can be assembled with alginate (ALG) through layer-by-layer deposition. Ellipsometry and fluorescence (FL) spectroscopy show an exponential growth of the TPE-CS/ALG multilayer films. Moreover, the exponential relationship between the FL intensity and the number of bilayers, which is in accordance with the thickness variation of multilayer films, provides solid evidence for its capacity to monitor the layer-by-layer self-assembly process.
